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Composite

Part:BBa_K781001

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-02)
Revision as of 13:57, 2 October 2012 by Registry (Talk | contribs)

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[R0010][B0034] - RFP-FliC Insertion Chimera

This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as an insertion.

This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
    Illegal AgeI site found at 938
    Illegal AgeI site found at 1552
    Illegal AgeI site found at 1664
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/a[R0010][B0034] - RFP-FliC Insertion Chimera