Coding

Part:BBa_K842007

Designed by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto   Group: iGEM12_USC   (2012-10-02)
Revision as of 01:15, 4 October 2012 by Rkohan (Talk | contribs)

cheB

USC cheb.gif

CheB removes the methyl group that are attached to the gamma-glutamyl methyl ester residues located in the methyl-accepting chemotaxis protein (MCP). This allows for the bacteria to remain alert to the changes in the environment.

Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None