Coding

Part:BBa_K842000

Designed by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto   Group: iGEM12_USC   (2012-10-02)
Revision as of 00:01, 4 October 2012 by Rkohan (Talk | contribs)

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FlhB is a Class 2 flagella operon. When transcribed, flhB is one of three flagella genes responsible for the generation of proteins that export flagellin to the flagella apparatus. The flagellin is then directly used in the synthesis of the structures’ basal body. Additionally, flhB directly transcribes the operon flhBAE.


FlhB was use generated via PCR from the e coli strain DH5α and cloned into the vector pSB1C3. It is directly downstream of the class 1 flagella operons flhD and flhC. It also works in conjunction with fliL and fliH to make up the flagellin export system. This part can be used either to generate a flagella in an e. coli strain in which they are not already present, or to promote further formation of pre-existing flagella.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None