Generator

Part:BBa_K801076:Design

Designed by: Dennis Hell   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 15:33, 3 October 2012 by Nadine1990 (Talk | contribs) (→‎Source)

Yeast expression cassette for CaXMT1 and CaMXMT1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3613
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385

Composite part of BBa_K801010, BBa_K801070, BBa_K801012, BBa_K319003, BBa_K801071 and BBa_K801012


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Design Notes

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Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBrick BBa_K801075 is further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

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Source

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