Tag

Part:BBa_K844000

Designed by: Kathleen Miller   Group: iGEM12_Utah_State   (2012-10-02)
Revision as of 17:28, 2 October 2012 by Skiracer96 (Talk | contribs)

10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part.


Usage and Biology

This part is a 10x-Histidine tag with two stop codons on the 3’ end. It is intended to be cloned downstream of a protein or protein subunit lacking a stop codon; the 10x-Histidine tag can then be used to extract the protein using Nickel-based affinity columns. Proteins on gels or in cells can also be analyzed with His-tag specific antibodies or dyes.

The 10x His tag conveys increased binding affinity for nickel resin compared to traditional 6x His tags as shown in [citation needed].

IMPORTANT NOTE: Any dyes, antibodies, affinity resins, etc. designed for use with 6x-Histidine tags will work with this 10x-Histidine tag, and in most cases should work better.


References

[citation needed]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None