DNA

Part:BBa_K887011

Designed by: Yu, CHIEH-CHENG   Group: iGEM12_NCTU_Formosa   (2012-09-16)
Revision as of 14:56, 2 October 2012 by Ez810711 (Talk | contribs)

DNA program : specific DNA sequence which can be recognized by zinc fingers

In order to promote the yield of isobutanol further, we not only use the temperature control system we designed but also encode three different kinds of zinc finger functional domain genes (zif268 : Part:BBa_K323105, PBSll : Part:BBa_K323107 and hivC : Part:BBa_K165006) preceding each enzyme genes.(More details about the biobrick, please refer to Part:BBa_K887000)

It means that these fusion proteins (combination of zinc finger functional domain and our enzymes) will bind specific DNA sequence and also carry out isobutanol biosynthesis reaction simultaneously.

The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize

Dpicture0.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aWe find that the original Slovenia DNA program part a base pair missed, and thus it will make the DNA framshift.