T7

Part:BBa_K921000

Designed by: Eric Pederson   Group: iGEM12_Carnegie_Mellon   (2012-10-01)
Revision as of 21:31, 1 October 2012 by Registry (Talk | contribs)

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T7 RNAP + IPTG->PoPs (Mutant I)

This is a mutant T7 promoter that includes a lacO sequence. This is repressible by the LacI protein and is induced in the presence of IPTG. The promoter sits in the 5' region of the gene of interest and initiates transcription when the cell expresses T7 RNA polymerase. Be sure you are using a cell strain that includes this gene (DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/bacteriophage/t7
//function/regulation/transcriptional
//plasmid/expression/t7
//promoter
//regulation/negative
Parameters
directionForward
efficiency169%
negative_regulatorsLacI (Hypothesized LacIQ compatible)