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Translational_Unit

Part:BBa_K917011:Design

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-24)
Revision as of 17:14, 24 September 2012 by Registry (Talk | contribs)

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lac promoter plus sucrose hydrolase (cscA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 873
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to the pathogenic nature of the source strain, purified genomic DNA was obtained from a veterinary laboratory to use as a PCR template, and the E. coli O157:H7 strain was never present in viable form in our laboratory. Note that the native RBS is included.


Source

Escherichia coli O157:H7 strain Sakai

References