Composite
Part:BBa_K917005:Design
Designed by: Elitsa Peeva Group: iGEM12_Edinburgh (2012-09-20)
lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1189
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part includes the native ribosome binding site. The original sequence (http://www.ncbi.nlm.nih.gov/nuccore/M63808.1) had a PstI restriction site however the cloned gene did not have PstI restriction site ( confirmed trough sequencing and restriction digest).
Source
Enterobacter cloacae genomic DNA