Generator

Part:BBa_K741003

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 17:23, 30 September 2012 by Dunkle (Talk | contribs) (zs)

pRM-RBS-GFP-T

Express GFP only if protein CI activated the promoter pRM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752

USTC2012Result2 副本.png

Figure 2 shows that the fluorescence intensity of prm-gfp is so weak in comparison with plac-gfp that we can assume that the leak expression of prm is very few, which doesn't affect our experiments.

Data

                                pRM-RBS-GFP-T
                                    OD
t/min Parallel Experimental group M9 medium
0 1 0.0593 0.045
  2 0.0642  
  3 0.0678  
45 1 0.0605 0.0436
  2 0.0734  
  3 0.062  
90 1 0.0648 0.0435
  2 0.0735  
  3 0.0702  
135 1 0.0811 0.0447
  2 0.0716  
  3 0.0677  
180 1 0.0723 0.0446
  2 0.0828  
  3 0.0789  
                                pRM-RBS-GFP-T
                                      fluorescence intensity
t/min Parallel Experimental group M9 medium
0 1 19.55 11.801
  2 19.571  
  3 19.352  
45 1 21.548 13.386
  2 20.505  
  3 19.107  
90 1 24.441 11.541
  2 23.777  
  3 24.503  
135 1 28.938 11.894
  2 27.884  
  3 27.81  
180 1 41.376 11.066
  2 39.573  
  3 39.795  
[edit]
Categories
Parameters
None