Part:BBa_K741012
plac-anticro-T -pCon(0.856)-RBS-crogfp-T
The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 124
Illegal BglII site found at 1481 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 918
To test the function of antisense RNA to cro (anticro) we have constructed, we measure the fluorescence intensity of plac-anticroT-pcon(0.856)-crogfp and plac-cI-prm-anticroT-pcon(0.856)-crogfp.
At the beginning, the unit fluorescence intensity of the experimental groups with glucose added is the lowest, because glucose promotes the growth of E.coli., which makes the rate of fluorescence divided by OD lower than the other two groups.
About 45 minutes later, the unit fluorescence intensity of experimental groups with glucose added is higher than the blank group, because glucose represses the expression of plac, which reduces the transcription of anticro. Therefore, the translation of crogfp is repressed less and the unit fluorescence intensity is higher.
On the contrast, IPTG activates plac, which increases the transcription of anticro. Anticro binds to the mRNA crogfp and represses its translation.
| None |