Part:BBa_K812130

Citrine reporter with a Kozak sequence for expression in Xenopus

Citrine gene flanked by a kozak sequence for eukaryotic expression in biobrick format. This is designed to be used in mammalian and ovarion expression systems. The backbone of the plasmid is pCS2+ with the CMV promoter is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a> and the J04450 is replaced by the mCitrine <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812030">BBa_K812030</a>, it is ready for immediate use in Xenopus.

</br>This part was characterized and the result is below.

Tadpoles have 4 days (near stage 46) and the expression of mCitrine is localized in tail's muscles. <img src="" alt="perdu" width="500px" />

</html>

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 667
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]