Composite
pBAD-GFP-c

Part:BBa_K845009:Design

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)
Revision as of 13:55, 30 September 2012 by Greghansen (Talk | contribs) (→‎Design Notes)

paraBAD_gfp_cyaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 500
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 344
    Illegal BamHI site found at 1538
    Illegal XhoI site found at 494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 500
    Illegal AgeI site found at 179
    Illegal AgeI site found at 484
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2172
    Illegal BsaI.rc site found at 1169
    Illegal SapI site found at 161
    Illegal SapI.rc site found at 1662
    Illegal SapI.rc site found at 1902


Design Notes

Our safety notes:

https://static.igem.org/mediawiki/2012/4/48/CyaA.pdf

https://static.igem.org/mediawiki/2012/2/2a/ParaBAD-gfp.pdf

Source

...

References