Primer
Part:BBa_K864445:Experience
Designed by: Arvid Hedén Gynnå, Sabri Jamal, Lisa Branzell, Hampus Elofsson Group: iGEM12_Uppsala_University (2012-09-29)
Revision as of 21:48, 29 September 2012 by Sabrijamal (Talk | contribs)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
The primers that were used in order to perform the site directed mutagenesis to replace the antisense region with a random nucleotide sequence showed to contain a problematic secondary structure. Be sure to check https://parts.igem.org/Part:BBa_K864440:Experience to see the PCR program and PCR protocol that was used to bypass the problems with the secondary structure.
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UNIQ8c2de9203ac8221a-partinfo-00000000-QINU UNIQ8c2de9203ac8221a-partinfo-00000001-QINU