Coding
SYFP2

Part:BBa_K864100:Experience

Designed by: Erik Gullberg   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 18:08, 29 September 2012 by Agynna (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K864100

User Reviews

UNIQ956e3e59b5df0b99-partinfo-00000000-QINU

No review score entered. User:agynna

iGEM Team Uppsala University 2012

This part was used as an fluorescent reporter in two ways by the Uppsala iGEM team 2012. It could easily be observed both in a Fluorescence activated cell sorter (FACS), on a UV table and even better on a Visi-Blue blue-light table. We recommend it whenever a strong fluorescent reporter is needed.

1. As a part of the sRNA screening system target construct (BBa_K864444), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS.

2. In our promoter test, where it was coupled to different promoter as promoter-B0032-SYFP2 (using BBa_K864101).

;


UNIQ956e3e59b5df0b99-partinfo-00000004-QINU