Composite
pBAD-GFP

Part:BBa_K845000:Experience

Designed by: Grégory Hansen   Group: iGEM12_Grenoble   (2012-09-26)
Revision as of 15:20, 29 September 2012 by Greghansen (Talk | contribs) (→‎Applications of BBa_K845000)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K845000

In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA. We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media: M9 complement with 0.03% of glucose and 0.03% of acetate (1) M9 complement with 0.1% of glycerol (2) M9 complement with 0.1% of acetate (3)

See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test

Those different media did not affect bacterial growth.

Characterization glucose.png

Characterization acetate.png

Characterization glycerol.png

User Reviews

UNIQ05e824eda18d84ca-partinfo-00000000-QINU UNIQ05e824eda18d84ca-partinfo-00000001-QINU