Measurement

Part:BBa_K772008:Design

Designed by: Mustafa Elitok   Group: iGEM12_Fatih-Medical   (2012-09-26)
Revision as of 23:50, 26 September 2012 by Registry (Talk | contribs)

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GFP - LacZ protein complex with flag tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693


Design Notes

Cleavage site, LacZ and GFP sequences are gathered from Registry.


Source

Note that there is an RBS sequence just before the protein sequence in order to eliminate the need of addition of it.

References