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Part:BBa_K914007

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-09-20)
Revision as of 23:20, 20 September 2012 by Registry (Talk | contribs)

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Meganuclease I-SceI controlled by pBad

I-SceI homing endonuclease expression is controlled by pBad promoter. Expression is induced with IPTG if LacI is present in the cell. I-SceI doesn't have an LVA degradation tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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