Coding
fhlA-E363

Part:BBa_K778002

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-22)
Revision as of 00:07, 27 September 2012 by YutaOtsuka (Talk | contribs)

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). However, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production. 

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None