Coding
Part:BBa_K909008
Designed by: Gintautas Vainorius Group: iGEM12_ETH_Zurich (2012-09-23)
tetR-DBD-UVR8 fusion protein
Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 902
Illegal PstI site found at 1556 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 418
Illegal PstI site found at 902
Illegal PstI site found at 1556 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 382
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 902
Illegal PstI site found at 1556 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 902
Illegal PstI site found at 1556 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 433
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Categories
Parameters
//cds/membrane/receptor
None |