DNA
Part:BBa_K863202:Design
Designed by: Julia Schirmacher Group: iGEM12_Bielefeld-Germany (2012-09-23)
his4 gene from Pichia pastoris
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2555
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1850
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 358 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2555
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2555
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 510
Design Notes
design considerations are coming soon
Source
The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.