DNA

Part:BBa_K863202:Design

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)
Revision as of 16:22, 23 September 2012 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


his4 gene from Pichia pastoris


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2555
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 510


Design Notes

design considerations are coming soon


Source

The DNA sequence from plasmid pPIC9K of Invitrogen (http://products.invitrogen.com/ivgn/product/V17520?ICID==%3Dsearch-product) was used for primer design. But the genomic DNA of Pichia pastoris wildtype X33 was used for amplification via PCR.


References