Composite

Part:BBa_K899004

Designed by: Andreas Kjær   Group: iGEM12_SDU-Denmark   (2012-09-22)
Revision as of 01:22, 27 September 2012 by Larsnorge (Talk | contribs)

Reporter system for SST and FFT (BBa_K899000 and BBa_K899001)

Reporter system to test for transcription of fructan:fructan fructosyltransferase (FFT) and Sucrose:sucrose fructosyltransferase (SST) genes with green flourescent protein (GFP) as reporter, and lacl as promoter.

This construct was designed with the promoter BBa_R0010 in order to control transcription. Transcription of 1-SST and 1-FFT greatly inhibit bacterial growth with the production of plant inulin. LacI protein and CAP can succesfully inhibit transcription of both genes to allow the bacteria to grow. With the addition of IPTG to the medium, LacI will be inhibited and transcription can resume.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2053
    Illegal BglII site found at 2662
    Illegal BamHI site found at 346
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 297
    Illegal AgeI site found at 2516
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4666


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