Coding

Part:BBa_K797003:Experience

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-20)
Revision as of 00:58, 27 September 2012 by Grassfield (Talk | contribs) (→‎Applications of BBa_K797003)

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Applications of BBa_K797003

To check whether the expression of kil affects the growth of cells or not, we used the construct, lacp-RBS-Kil-double terminator. We did the experiment as this protocol, and we got the following data. Protocol

  1. Grow cells at 37 degrees for 18h.
  2. Dilute it until OD600=0.1.
  3. Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
  4. Incubate the cultures at 30 degrees and measure OD600 of them every 30 min

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