Composite

Part:BBa_K953000

Designed by: Ryan Kenny   Group: iGEM12_Macquarie_Australia   (2012-09-20)
Revision as of 00:43, 27 September 2012 by DanWinter (Talk | contribs)

Heme Oxygenase with T7 & RBS (E. coli Codon Optimised)

Synechocystis Heme Oxygenase with a RBS and codon optimised for expression in E. coli. This part differs from Part BBa_K953004 in that it also includes a T7 promoter (Part BBa_I712074). , oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

[edit]
Categories
Parameters
n/aHeme Oxygenase with T7 & RBS (E. coli Codon Optimised)