Part:BBa_K801001:Design

pTUM101 yeast shuttle vector with pTEF1 promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 36
  Illegal suffix found at 506
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  Illegal NheI site found at 3771
  Illegal SpeI site found at 507
  Illegal PstI site found at 521
  Illegal NotI site found at 42
  Illegal NotI site found at 514
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 36
  Illegal suffix found at 507
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 36
  Plasmid lacks a suffix.
  Illegal XbaI site found at 51
  Illegal SpeI site found at 507
  Illegal PstI site found at 521
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 261
  Illegal BsaI.rc site found at 1928
  Illegal BsaI.rc site found at 2874
  Illegal SapI site found at 845
  Illegal SapI site found at 3026
  

All forbidden restriction sites have been removed from the plasmid backbone, making it RFC25 compatible

Based on invitrogen's pYES2 plasmid. For more information see the part information of pTUM100 (BBa_K801000).

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

• K801000: BBa_K801001
• K801002: BBa_K801002
• K801003: BBa_K801003

Quality control measures:
Test digestion using SpeI+ PstI

Backbone:
Resistance: Amp

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2, Invitrogen

Organism:
Designed for the following Chassis:Saccharomyces cerevisiae, Escherichia coli

References

Literature references:

Database references: