Coding

Part:BBa_K801080:Design

Designed by: Alois Bruer   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 17:24, 26 September 2012 by Mary (Talk | contribs) (→‎Design Notes)

Prepro-Thaumatin, yeast codon optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Synthesized by IDT. The part is codon optimized for yeast. 3 forbidden restriction sites have been removed.

Please note, that the pre- and pro-part are split off, thus there is no possibility to add streptags or form other recombinant proteins.


Based on: http://www.ncbi.nlm.nih.gov/nuccore/121945717



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Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick leads to the production of thaumatin. We investigated the toxicity of thaumatin and incubated yeast cells (strain INVSc) with 45 mM of thaumatin. We found out, that thaumatin does not show any negative effect on the growth rate of yeast cells.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

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References

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