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Part:BBa_K784021

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-17)
Revision as of 16:45, 21 September 2012 by Hila K R (Talk | contribs)

Phage Lambda fragment 7 - replication and lytic regulation

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
Fragment 7 contains the coding regions for the phages replication proteins O and P and the coding sequence for the lytic cycle regulatory protein Q.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal BglII site found at 275
    Illegal BglII site found at 335
    Illegal BamHI site found at 3253
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 689
    Illegal EcoRI site found at 6493
    Illegal AgeI site found at 1973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4236


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