Reporter
Part:BBa_K819006:Design
Designed by: YU Zhou Group: iGEM12_Peking (2012-09-02)
Testing device for Luminesensor
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 839
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 839
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 839
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 839
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 734
Design Notes
GFP ligated to the rear of a sulA promoter in 408 form (only recognizable by our LexA-VVD fusing protein, not by E.coli endogenous LexA).
Source
GFP with ssrA tag was from fromer Peking iGEM team members.