Reporter

Part:BBa_K819006:Design

Designed by: YU Zhou   Group: iGEM12_Peking   (2012-09-02)
Revision as of 03:13, 3 September 2012 by Registry (Talk | contribs)

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Testing device for Luminesensor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 839
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 839
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 839
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 734


Design Notes

GFP ligated to the rear of a sulA promoter in 408 form (only recognizable by our LexA-VVD fusing protein, not by E.coli endogenous LexA).


Source

GFP with ssrA tag was from fromer Peking iGEM team members.

References