Part:BBa_K896001

CysI (Sulfite reductase)

Our engingeered cyanobacteria can remove Sulfur Oxides (SOX, SO2),main precursors of air pollution and produce H2S for futher uses(Sulfide-Quinone Reductase,sqr).

CysI( surfur reductase) :This is a enzyme that can reduce (HSO3)- to H2S using the embedded electron transfer system in pseudomonas aeruginosa PAOI.

[http://www.rcsb.org/pdb/explore/explore.do?structureId=3MM5 sulfite reductase in complex with the substrate sulfite]

Usage and Biology

1. From clinical research, we fund out that Pseudomonas aeruginosa can produce great amount H2S.So, we logical assumed there are some pivotal enzymes can transfer (HSO3)- to H2S.

2. We searched for [http://www.genome.jp/kegg/ KEGG] and [http://www.ncbi.nlm.nih.gov/ NCBI] and found out CysI, a sulfite reductase inside [http://www.ncbi.nlm.nih.gov/protein/NP_250529.1 Pseudomonas aeruginosa PAOI].

3.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 655
  Illegal PstI site found at 121
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 655
  Illegal PstI site found at 121
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 655
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 655
  Illegal PstI site found at 121
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 655
  Illegal PstI site found at 121
  Illegal NgoMIV site found at 394
  Illegal NgoMIV site found at 580
  Illegal NgoMIV site found at 722
  Illegal AgeI site found at 94
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 130
  Illegal BsaI site found at 1452
  

Functional Parameters

kegg	Pseudomonas aeruginosa PAO1: PA1838