Generator
Part:BBa_K819005
Designed by: ZHANG Zidong Group: iGEM12_Peking (2012-09-02)
Revision as of 16:18, 26 September 2012 by Zhangzidong (Talk | contribs)
Constitutive LuxBrick Generator
This part serves as an ultra sensitive photoreceptor (which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoters like RecA408 promoter and SulA408 promoter.
It is a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass light sensing protein VVD. Both the LexA domain and VVD domain of this protein were mutated at specific sites for better performance. (See the design page for details, please click here)
unfinished...
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726
[edit]
Categories
Parameters
//cds
//chassis/prokaryote/ecoli
//function/cellsignalling
//chassis/prokaryote/ecoli
//function/cellsignalling
emission | maxnium at 485nm |
n/a | Constitutive LuxBrick generator |