Generator
Part:BBa_K819005
Designed by: ZHANG Zidong Group: iGEM12_Peking (2012-09-02)
Revision as of 15:39, 26 September 2012 by Zhangzidong (Talk | contribs)
Constitutive LuxBrick Generator
It is a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass light sensing protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes under specific promoter in 408 form.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726
[edit]
Categories
Parameters
//cds
//chassis/prokaryote/ecoli
//function/cellsignalling
//chassis/prokaryote/ecoli
//function/cellsignalling
emission | maxnium at 485nm |
n/a | Constitutive LuxBrick generator |