Coding
CysI
Part:BBa_K896001
Designed by: Shih-Hao Chen, Yen-Chen Lo,Ting-Yi Lin Group: iGEM12_NYMU-Taipei (2012-09-21)
CysI (Sulfite reductase)
Our engingeered cyanobacteria can remove Sulfur Oxides (SOX, SO2),main precursors of air pollution and produce H2S for futher uses(Sulfide-Quinone Reductase,sqr).
[http://www.rcsb.org/pdb/explore/explore.do?structureId=3MM5 sulfite reductase in complex with the substrate sulfite]
CysI( surfur reductase) :This is a enzyme that can reduce (HSO3)- to H2S using the embedded electron transfer system in pseudomonas aeruginosa PAOI.
Usage and Biology
1. From clinical research, we fund out that Pseudomonas aeruginosa can produce great amount H2S.So, we logical assumed there are some pivotal enzymes can transfer (HSO3)- to H2S.
2.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 655
Illegal PstI site found at 121 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 655
Illegal PstI site found at 121 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 655
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 655
Illegal PstI site found at 121 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 655
Illegal PstI site found at 121
Illegal NgoMIV site found at 394
Illegal NgoMIV site found at 580
Illegal NgoMIV site found at 722
Illegal AgeI site found at 94 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 130
Illegal BsaI site found at 1452
Functional Parameters
kegg | Pseudomonas aeruginosa PAO1: PA1838 |
[edit]
Categories
Parameters
kegg | Pseudomonas aeruginosa PAO1: PA1838 |