Translational_Unit

Part:BBa_K812057

Designed by: Hafez El Sayyed   Group: iGEM12_Evry   (2012-09-26)
Revision as of 15:44, 26 September 2012 by Hafez (Talk | contribs)

A GoldenBricked version of sfGFP protein 

SfGFP  designed to fit the Golden brick assembly protocol as a reporter that we have used to test the Goldenbrick system. On top of this, the SfGFP belongs to the family of the famous GFP family of fluorescent reporters that fits any detection purpose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10
    Illegal BsaI.rc site found at 748
    Illegal SapI.rc site found at 33


[edit]
Categories
Parameters
None