Part:BBa_K737067
pLux OmpR hybrid promoter
In order to optimize the function of the lux promoter, We creat a hybrid promoter which combine plux with OmpR, and in this case, plux can be lead to expression by AHL and obtain by phosphorylation of OmpR protein. According to the research of it before, we camp up with a program . Keep the sequencing of Lux Box, -35box and -10box. Then put the sequencing of C3 point of OmpR promoter into -35box and -10box.
Figure structural representation of Plux promoter
Figure : the analysis of conserved sequence of pLux by Weblogo
Plux promoter is organized from the promoter the shining gene of V. fischeri(R0062). Through analyzing common hybrid promoter of Lux by Weblogo , we found that the sequence of Lux Box is conserved. In benefit of LuxR Binding Site, -35box and -10box of the promoter the shining gene of V. fischeri(R0062).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| n/a | OmpR |