Part:BBa_K782085
10x[TALA] operator_minimal promoter_TALA:NLS:VP16_t2a_BFP
TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011)
Introduction
Our construct contain TALA binding sites that are cloned upstream of minimal promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results: Specific TAL binding sites were further characterized with other reporter constructs.
BFP was obtained from Evrogen.
References
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 750
Illegal BamHI site found at 720
Illegal BamHI site found at 3262
Illegal XhoI site found at 788 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 507
Illegal AgeI site found at 12
Illegal AgeI site found at 347
Illegal AgeI site found at 372
Illegal AgeI site found at 707 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4207
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