Device

Part:BBa_K786003

Designed by: Leung Wai Tak   Group: iGEM12_Hong_Kong-CUHK   (2012-09-21)
Revision as of 14:00, 21 September 2012 by Registry (Talk | contribs)

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Sensory rhodopsin I (SRI) with HtrII & Tar, sensitive to orange light

Sensory rhodopsin I fusion with HtrII & Tar, which is sensitive to orange light, with sensing spectra covering from 590-650 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 37
    Illegal NgoMIV site found at 614
    Illegal NgoMIV site found at 638
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 875
    Illegal AgeI site found at 144
    Illegal AgeI site found at 1616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 977


[edit]
Categories
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//classic/signalling/sender
//function/sensor/light
//plasmid/expression
Parameters
device_type
function
protein