Reporter
beta-galac

Part:BBa_K823019:Design

Designed by: Simon Kelterborn   Group: iGEM12_LMU-Munich   (2012-08-15)
Revision as of 15:33, 15 August 2012 by Registry (Talk | contribs)

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LacZ (RBS+Freiburg standard)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed the part in the Freiburg standard (assembly 25) for inframe fusion-proteins. There is a Shine-Dalgarno Sequence included between the XbaI and the NgoIV restriction site. It can be used for both N and C terminal fusions.


Source

Bacillus subtilis reporter vector pAC6.

References