Composite

Part:BBa_K823040:Design

Designed by: Julia Bartels   Group: iGEM12_LMU-Munich   (2012-09-10)
Revision as of 09:48, 10 September 2012 by Registry (Talk | contribs)

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Inverter: pBad-RyhB-pLac(R0011)-uof-lacZalpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Fusion of the small RNA directly to promotor to ensure exact +1 site and length. Translational fusion of uof to lacZalpha. These had to be done by fusionPCRs


Source

E.coli, Plasmids

References