Part:BBa_K782068
4x[NicTAL]+4x[TALD] operator
- TALD label represents TAL effector 1295 from zebrafish experiments (Sander et al., 2011).
Introduction
Introduction
Our construct contains four specific binding sites for NicTAL12 and TALD.
Single binding sequence for NicTAL12 is: TCTATCAATGATAGA
Single binding sequence for TALD is: TCGTCCAATAGCTTCTC
Figure 1. Shematic representation of four consecutive specific binding sites for NicTAL12 and TALD.
Characterization
Results: Specific TAL binding sites were further characterized with different reporter constructs.
- Binding sites for TAL effectors were ordered from IDT.
References
Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 35
Illegal AgeI site found at 235 - 1000COMPATIBLE WITH RFC[1000]
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