Generator
LacIM

Part:BBa_K819012

Designed by: ZHANG Hong   Group: iGEM12_Peking   (2012-09-03)
Revision as of 04:37, 26 September 2012 by Zhangzidong (Talk | contribs)

Luminesensor repressible LacIM generator

LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor BBa_K819005 constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//function/cellsignalling
Parameters
n/aLuminesensor repressible LacIM generator