Generator
LacIM
Part:BBa_K819012
Designed by: ZHANG Hong Group: iGEM12_Peking (2012-09-03)
Revision as of 04:37, 26 September 2012 by Zhangzidong (Talk | contribs)
Luminesensor repressible LacIM generator
LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor BBa_K819005 constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//function/cellsignalling
//chassis/prokaryote/ecoli
//function/cellsignalling
n/a | Luminesensor repressible LacIM generator |