Coding

Part:BBa_K896000

Designed by: Hsun-I Chiu   Group: iGEM12_NYMU-Taipei   (2012-09-09)
Revision as of 03:22, 26 September 2012 by Cutelucypoop (Talk | contribs)

SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7 

 SQR introduction:
Sulfide-dependent anoxygenic photosynthesis, driven by photosystem I (PS/I) alone, among cyanobacteria was first described for Oscillatoria limnetica from Solar Lake. Later photosynthetic sulfide oxidation in O. limnetica led to the discovery of sulfide-quinone reductase (SQR; E.C.1.8.5.′), a novel enzyme that transfers electrons from sulfide into the quinone pool.

Above are sulfide-induced sulfide-Quinone Reductase with the electron transport system.

SQR ETC.png(Cohen, Y., E. Padan, and M. Shilo, Facultative anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. J Bacteriol, 1975. 123(3): p. 855-61.) 


 Cloning of SQR gene:
SQR gene came from Synechococcus elongatus PCC7002 because the hypersaline strain S. elongatus PCC 7002, which is already sequenced, is 96% similar to O.limnetica SQR gene.

Cloning SQR.jpg 

 Function analysis of SQR gene:


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 349


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Parameters
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