Signalling

Part:BBa_K819015

Designed by: YU Zhou   Group: iGEM12_Peking   (2012-09-16)
Revision as of 22:26, 25 September 2012 by Zhangzidong (Talk | contribs)

Luminesensor repressible RecA408 promoter+CheZ

CheZ is a significant protein in chemotaxis pathway of E.coli. It can prevent the cells from tumbling around and keep the cells moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ placed under RecA 408 promoter (see BBa_K819002 constructed by Peking 2012 iGEM) can be repressed by LexA 408 protein or protein with LexA 408 domain(e.g. optimized luminesensor BBa_K819005 constructed by Peking 2012 iGEM team), excluding the influence of endogenous LexA, forming an orthogonal modular biobrick. Thus the motility of E.coli can be regulated

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//chassis/prokaryote/ecoli
//function/cellsignalling
Parameters
n/aLuminesensor repressible recA408 promoter+CheZ
targetCheY