Measurement

Part:BBa_K911003:Design

Designed by: Oliver Meacock   Group: iGEM12_Cambridge   (2012-09-12)
Revision as of 16:32, 22 September 2012 by Olijme (Talk | contribs) (→‎Source)

Fluoride Sensitive Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 26
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ was used as our reporter.

Source

  • Bacillus Cereus Genome
  • Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.

References

  • Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.