Other
Polyargini

Part:BBa_K792004

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-24)
Revision as of 07:21, 25 September 2012 by Manugimenez (Talk | contribs)

Import enhancer - Polyarginine (trojan peptide)


Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.


This part is Polyarginine, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.


This part includes a HindIII restriction site as an extra.


(Refer to Characterisation of cell-penetrating peptide-mediated peptide delivery [Jones et al 2005] for more information about polyarginine)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/internal/special
Parameters
None