Composite

Part:BBa_K929004:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)
Revision as of 09:59, 25 September 2012 by ChristopherKressler (Talk | contribs) (→‎Design Notes)

modified AID+eGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 1225
    Illegal SapI site found at 209


Design Notes

UP 12 designinfo BBa K929004.png

The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. The resulting Biobrick (BBa_K929004) is in RFC 10 and has also an aditional AgeI restriction site in front of the stop codon taa. Therefore further fusion(C-terminal of (BBa_K929004 with RFC 25 parts is possible.

Source

This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID).

References