Signalling
Part:BBa_K750000
Designed by: Sifan Wang, Yizhen Yan, Jianzhao Chi, Qingshu Wu Group: iGEM12_XMU-China (2012-09-18)
pBADGLT:unstable gfp expression device activated by arabinose
Description
This part contains Pbad promoter, RBS of 1.0 strength, GFP(lva) producer and double terminator. GFP(lva) will be produced after arabinose added. We use this part in our 0,1 dispaly system.
Performance
After completing the construction of this device, we did fluorescence test and protein electrophoresis of gfp(lva). Here is the electrophoresis image of gfp(lva).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 800
[edit]
Categories
Parameters
control | K206000 |
device_type | signalling |
proteins | GFP(lva) |