Generator

Part:BBa_K792012

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-25)
Revision as of 14:57, 25 September 2012 by Manugimenez (Talk | contribs)

Yeast exportable Trp-rich peptide w/enhanced import (2)

This composite device produces and exports a Tryptophan rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.


The high level structure of the device is:

  • BamHIrestriction site
  • Kozak consensus sequence for initiation of translation
  • Signal peptide that targets the product of the gene for secretion
  • Trojan peptide, to increase internalization in target cell (Polyarginine)
  • Payload: this is the exported Tryptophan rich domain of the protein (a custom designed peptide that we called PolyWb)


The device's sequence has inherited some useful restriction sites (BamHI & HindIII) from the parts it's made of.


Important note: this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None