Signalling
TD1.0

Part:BBa_K750009:Design

Designed by: Sifan Wang,Ruosang Qiu,Xinyi Yao,Qingshu Wu   Group: iGEM12_XMU-China   (2012-09-19)
Revision as of 13:56, 25 September 2012 by AlexYao (Talk | contribs) (→‎Source)

TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1069
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 793
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1009
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2024
    Illegal BsaI.rc site found at 2751


Design Notes

editing


Source

We built this part by biobricks from the DNA distribution kit plates 2012. The inspiration of design this part comes from further research of the iccdB project we designed last year.

References

1. W.Claiborne Fuqua, S.C.W., E. Peter Greenberg, Quorum Sensing in Bacteria: the LuxR-LuxI Family of Cell Density-Responsive Transcriptional Regulatorst. JOURNAL OF BACTERIOLOGY, 1994. 176(2): p. 269-275.
2. You, L., et al., Programmed population control by cell-cell communication and regulated killing. Nature, 2004. 428(6985): p. 868-71.
3. Alon, U., Network motifs: theory and experimental approaches. Nat Rev Genet, 2007. 8(6): p. 450-61.
4. Mangan, S., et al., The incoherent feed-forward loop accelerates the response-time of the gal system of Escherichia coli. J Mol Biol, 2006. 356(5): p. 1073-81.
5. Camas, F.M., J. Blazquez, and J.F. Poyatos, Autogenous and nonautogenous control of response in a genetic network. Proc Natl Acad Sci U S A, 2006. 103(34): p. 12718-23.
6．The project iccdB of team XMU-China in iGEM 2011: http://2011.igem.org/Team:XMU-China