Protein_Domain
Part:BBa_K747002
Designed by: Lucas Schneider Group: iGEM12_Freiburg (2012-09-24)
TAL-Protein_AG1_Direpeat
Pipette the following volumes in one tube:
Component | Volume |
6 Direpeats of your choice | 63,3 ng |
Backbone e. g. TAL-TF | 1,5 |
BsmBI | 1,5 |
T4 DNA Ligase (60 u) | 1,0 |
T4 DNA Ligase Buffer | 2,0 |
Water, nuclease-free | up to 20µl |
Total vloume | 20 µl |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//proteindomain/dnabinding
None |