Coding

Part:BBa_K883161

Designed by: Hugo de Vries   Group: iGEM12_Wageningen_UR   (2012-09-24)
Revision as of 14:00, 24 September 2012 by Registry (Talk | contribs)

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CCMV_Negative core antigen with IPTG induced promotor

This construct produces CCMV core antigen subunits. The subunits have been modified in such a way that they will fold into CCMV Virus-Like Particles that have a negative interior. This was done by replacing 6 Arginine and 3 Lysine residues at the N-Terminus with Glutamic acid residues. The promotor and RBS that have been added to the gene allow induced expression of the subunits which increases the yield. The full protocol for this construct can be found on the Wageningen_UR 2012 Wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 370
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None